User talk:Firdoshrm

Hello Firdoshrm,

I wanted to let you know that I just tagged Cellugen biotech for deletion, because it seems to be promotional, rather than an encyclopedia article.

If you feel that the article shouldn't be deleted and want more time to work on it, you can contest this deletion, but please don't remove the speedy deletion tag from the top.

You can leave a note on my talk page if you have questions. Cotton2 (talk) 12:46, 12 October 2016 (UTC)[reply]

Welcome to Wikipedia. A page you recently created may not conform to some of Wikipedia's guidelines for new pages, so it will be removed shortly (if it hasn't been already). Please use the sandbox for any tests, and consider using the Article Wizard. For more information about creating articles, you may want to read Your first article. You may also want to read our introduction page to learn more about contributing. Thank you. Stephen! ^Coming... 12:58, 12 October 2016 (UTC)[reply]

Adult Stem Cells in Regenerative Medicine

The interest in stem cells for tissue engineering and regenerative medicine has been increased because of properties possessed by stem cells such as proliferation potential, multi lineage differentiation potential and capacity to modulate immune phenotype. Mesenchymal stem cells are gaining popularity as cell therapeutic option for treating various disorders. Stem cell therapies are promising strategies to regenerate injured tissues caused by diseases or trauma.

A major constrain to stem cell therapeutic to clinical application is the supply of adequate number of cells. Mesenchymal stem cells are routinely expanded on 2D culture plates but with concerns of reduced clonogenicity and differentiation capacity after few passages. To maintain their unique properties, stem cells are needed to be grown in tissue like environment. 3D scaffolds provide a better resemblance of natural environment which can hardly be represented by 2D plastic substrate. 3 D microporous and nanofibrous scaffolds will be produced with freeze drying and electrospinning process respectively. As prepared scaffolds will be biomimetic and resemble the extra cellular matrix of organs, stem cells will adhere and proliferate well on scaffolds. The cell scaffold constructs can find its application in delivering the stem cells at target site.

Stem Cells in Tissue Engineering

Burns are major growing problem worldwide and there is need to develop effective treatment strategy to heal burns. In India, majority of surgeons are using collagen sheets to treat burns due to its low cost and associated advantages with it. Bone marrow is majorly known to responsible for delivering the progenitor and stem cells in response to injury. Bone marrow derived mesenchymal stem cells are resources of autologus and allogeneic cell therapies in regenerative medicine. If the bone marrow stem cells are seeded on collagen sheets and use this stem cell loaded collagen sheet construct for treating burns and wounds, will provide efficient and faster wound healing.

Umbilical Cord Blood in Transfusion Medicine

The advantages associated with umbilical cord blood are easy collection process, non invasive and less expensive method. MSCs derived from umbilical cord blood are non-immunogenic in nature, very few chances of rejection are there.

Blood cells are produced by proliferation and differentiation of a very small population of pluripotent hematopoietic stem cells (HSCs) that also have the ability to replenish themselves by self-renewal and differentiation into all blood cell types. Cord blood is a valuable source of hematopoietic stem cells and alternative for bone marrow transplantation. The application of cord blood cells for transplantation is often limited by low amount of CD34+ haematopoetic stem cells. HSCs do not grow as autonomous units; they are surrounded by micro-environment and growth factors. Many efforts have been done to expand cells in order to retain stemness versus differentiation with use of either cytokines or stromal cells, or their combination. Previous studies combining MSCs, cytokine with HSCs has proven the enhanced CD34+ cells expansion. Therefore, by combining these two sources together may have better characteristics for therapeutic potential. — Preceding unsigned comment added by 122.176.101.140 (talk) 14:10, 12 October 2016 (UTC)[reply]

Advancements in medicine led to development of stem cell based therapy in which stem cells are administered to patients to achieve functional restoration of damaged tissues. Stem cells derived either from embryo or adult tissues hold great promise for regenerative medicine because of their self renewal and multi lineage differentiation potential. The associated moral and ethical concerns with embryonic stem cells limit its application in spite of higher regeneration potential. Mesenchymal stem cells obtained from adult tissues are getting attention for cell based therapeutics because of their ethical acceptance, non invasive, non tumorigenic, immunosuppressive nature and ability to differentiate into tissue specific cell types. Mesenchymal stem cells can be isolated from various tissues such as adipose tissue, bone marrow, dental pulp, umbilical cord etc.

Umbilical cord is attractive and abundant source of stem cells because of associated advantages such as non-invasive collection, high proliferation potential and better tolerance of HLA mismatch. To evaluate potential of MSC based cell therapy for a range of conditions, many clinical trials are on going and numbers of clinical trials are continuously increasing. Mesenchymal stem cells have displayed its efficiency in treating many diseases including both immune and non-immune diseases including Alzheimer disease, myocardial infarction, osteoarthritis, muscular dystrophy, multiple sclerosis, spinal cord injury etc. Research is continuously going on to have better knowledge on optimal route of administration, best engraftment time and fate of cells after administration. The safety, reproducibility, and quality of stem cells must be thoroughly examined before their clinical use. — Preceding unsigned comment added by 122.176.101.140 (talk) 06:36, 3 November 2016 (UTC)[reply]