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Reactive and conjugated dyes

Dye	Ex (nm)	Em (nm)	MW	ε(λmax)	QY	BI	Notes
Alexa Fluor 350	346	442	410.35	19,000
Alexa Fluor 405	402	421	1028.3	35,000
Alexa Fluor 430	434	539	701.7537	15,000
Alexa Fluor 488	495	519	884.91	73,000	0.92	67.16
Alexa Fluor 514	518	540	713.69	80,000
Alexa Fluor 532	531	554	812.88347	81,000	0.61	49.41
Alexa Fluor 546	556	573	1159.6	112,000	0.79	88.48
Alexa Fluor 555	555	565	~1250	155,000	0.1	15.5
Alexa Fluor 568	578	603	791.8	88,000	0.69	60.72
Alexa Fluor 594	590	617	819.85	92,000	0.66	60.72
Alexa Fluor 610	612	628	1284.8	144,000
Alexa Fluor 633	632	647	~1200	159,000
Alexa Fluor 635	633	647		140,000
Alexa Fluor 647	650	668	~1250	270,000	0.33	89.1
Alexa Fluor 660	663	690	~1100	132,000	0.37	48.84
Alexa Fluor 680	679	702	~1000	183,000	0.36	65.88
Alexa Fluor 700	702	723	~1400	205,000	0.25	51.25
Alexa Fluor 750	749	775	~1300	290,000	0.12	34.8
Alexa Fluor 790	782	805	~1750	260,000
APC (Allophycocyanin)	650	660	104 k	700,000 ^[1]	0.68 ^[2]	476
Aminocoumarin	350	445	330				Succinimidyl ester
Cy2	489	506	869.95	0.12	150,000	18
Cy3	548	562	765.9	0.04	150,000	6	Lifetime <0.3ns
Cy3B	558	572	658	0.7	130,000	91	Lifetime 2.8ns
Cy3.5	581	596	1102.4	0.14	120,000	16.8	Lifetime 0.5ns
Cy5	646	664	791.9	0.27	250,000	67.5	Lifetime 1.0ns
Cy5.5	673	692	1128.4	0.23	190,000	43.7	Lifetime 1.0ns
Cy7	747	774	818	0.28	250,000	70
SeTau-380-NHS	270;(381)	480	472	Succinimidyl ester; QY 0.56; Lifetime 32.5 ns
Hydroxycoumarin	325	386	331	Succinimidyl ester
Methoxycoumarin	360	410	317	Succinimidyl ester
Cascade Blue	(375);401	423	596	Hydrazide
Pacific Blue	403	455	406	Maleimide
Pacific Orange	403	551
SeTau-405-NHS	405	518	549	Succinimidyl ester; QY 0.80; Lifetime 9.3 ns
SeTau-405-Maleimide	405	518	574	Maleimide; QY 0.51; Lifetime 9.1 ns
Lucifer yellow	425	528
SeTau-425-NHS	425	545	543	Succinimidyl ester; QY 0.39; Lifetime 26.2 ns
NBD	466	539	294	NBD-X
R-Phycoerythrin (PE)	480;565	578	240 k
Seta-PerCP-680	480;(673)	676		Tandem dye
PE-Cy5 conjugates	480;565;650	670		aka Cychrome, R670, Tri-Color, Quantum Red
PE-Cy7 conjugates	480;565;743	767
Red 613	480;565	613		PE-Texas Red
PerCP	490	675	35kDa	Peridinin chlorophyll protein
TruRed	490,675	695		PerCP-Cy5.5 conjugate
FluorX	494	520	587	(GE Healthcare)
Fluorescein	495	519	389	FITC; pH sensitive
BODIPY-FL	503	512
Seta-555-NHS	555	570	986	Succinimidyl ester; QY 0.70
Seta-555-Azide	555	570	986	Azide; QY 0.70
Seta-555-DBCO	555	570	986	DBCO derivative; QY 0.70
Seta-R-PE-670	556;(653)	671		Tandem dye
Seta-580-NHS	578	602	632	QY 0.55
SeTau-647-NHS	649	695	1461	QY 0.61; Lifetime 3.2 ns
Seta-APC-780	651	780
Seta-680-NHS	678	698	1128	QY 0.24
TRITC	547	572	444	TRITC
X-Rhodamine	570	576	548	XRITC
Lissamine Rhodamine B	570	590
Texas Red	589	615	625	Sulfonyl chloride
APC-Cy7 conjugates	650;755	767		Far Red
Seta-780-NHS	777	807	1232	Far Red

Abbreviations:
Ex (nm): Excitation wavelength in nanometers
Em (nm): Emission wavelength in nanometers
MW: Molecular weight
ε(λmax): Extinction Coefficient at the wavelength of maximum absorption
QY: Quantum yield
BI: Brightness Index - A measure of relative intensity defined by the equation: BI = (ε(λmax)*QY)/1000

[1] ttps://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/spectral-data-for-b-pe-r-pe-and-apc.html

[2] ttps://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/spectral-data-for-b-pe-r-pe-and-apc.html

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