User:Biochem312/sandbox
Structure
Gelonin is a 30 kDa protein.[1] Gelonin is a dimer, consisting of two identical monomers. Each monomer is comprised of 251 amino acids, for a total of 502 residues. Gelonin is classified as an (α + β) protein, as its secondary structure consists of both beta sheets and alpha helices. Each monomer’s first 100 amino acids form 10 beta sheets, while their last 151 amino acids form 10 alpha helices. Gelonin’s two dimers are stabilized by hydrophobic interactions and hydrogen bonds. Specifically, the Asn22, Arg178, Asn180, and Lys237 residues of each monomer hydrogen bond with each other to stabilize the molecules. Likewise, the hydrophobic residues Tyr14, Ile15, Val16 and Pro38 from one monomer form hydrophobic interactions with the same residues in the adjacent monomer to further stabilize the dimer.[2]
Active Site
Gelonin’s active site is a cleft formed by six key residues: Tyr74, Gly111, Tyr113, Glu166, Arg169, and Trp198. The shape of the active site is stabilized by hydrogen bonding between Gly111 and Tyr113. Tyr113, Glu166, and Arg169 residues in the activate site participate in the enzymatic removal of adenine at the 4324 site of eukaryotic 28S rRNA.[2] Although the reaction mechanism of Gelonin has yet to be characterized in detail, it is believed to take place in a manner that is conserved among other Type 1 ribosome-inactivating proteins.[3] According to research performed on other Type 1 RIPs, Tyr113 and Arg169 form hydrogen bonds with nitrogen atoms in the adenine nucleobase. This facilitates the cleavage of the glycoside bond connecting the nucleobase and ribose, creating a transition state with a positively charged oxycarbonium ion intermediate. The oxycarbonium ion is stabilized by the negative charge of Glu166. Gelonin’s active site also contains three water molecules, which act as nucleophiles and attack the oxycarbonium ion, completing the reaction.[4]
Therapeutic Applications
Because of its ability to inhibit translation by cleaving eukaryotic 28S rRNA, gelonin has the potential to be utilized as a cancer therapy. The anticancer activity of gelonin has been demonstrated in numerous in vitro models.[5] However, because of its hydrophilicity, gelonin is unable to internalize in cells. This has made clinical applications of the macromolecule difficult.[6] Multiple gelonin delivery systems have been engineered, including conjugation to a cell-penetrating peptide [7], vesicle encapsulation using listeriolysin O[6], and attachment to bispecific antibodies[8]. All of these delivery systems have been shown to significantly decrease tumor size in vivo in a number of different cell lines. However, clinical trials for gelonin have yet to be authorized.
- ^ Rosenblum, Michael G.; Kohr, William A.; Beattie, Kenneth L.; Beattie, Wanda G.; Marks, William; Toman, P. David; Cheung, Lawrence (1 June 1995). "Amino Acid Sequence Analysis, Gene Construction, Cloning, and Expression of Gelonin, a Toxin Derived from Gelonium multiflorum". Journal of Interferon & Cytokine Research. 15 (6): 547–555. doi:https://doi.org/10.1089/jir.1995.15.547.
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- ^ a b Hosur, M. V.; Nair, Bindu; Satyamurthy, P.; Misquith, S.; Surolia, A.; Kannan, K. K. (14 July 1995). "X-ray Structure of Gelonin at 1.8 Å Resolution". Journal of Molecular Biology. 250 (3): 368–380. doi:https://doi.org/10.1006/jmbi.1995.0383.
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- ^ Stirpe, Fiorenzo; Barbieri, Luigi (20 January 1986). "Ribosome-inactivating proteins up to date". FEBS Letters. 195 (1–2): 1–8. doi:https://doi.org/10.1016/0014-5793(86)80118-1.
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- ^ Ren, Jingshan; Wang, Yaopin; Dong, Yicheng; Stuart, David I. (1 January 1994). "The N-glycosidase mechanism of ribosome-inactivating proteins implied by crystal structures of α-momorcharin". Structure. 2 (1): 7–16. doi:https://doi.org/10.1016/S0969-2126(00)00004-6.
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- ^ Kornberger, Petra; Skerra, Arne (1 March 2014). "Sortase-catalyzed in vitro functionalization of a HER2-specific recombinant Fab for tumor targeting of the plant cytotoxin gelonin". mAbs. 6 (2): 354–366. doi:https://doi.org/10.4161/mabs.27444.
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- ^ a b Provoda, Chester J.; Stier, Ethan M.; Lee, Kyung-Dall (12 September 2003). "Tumor Cell Killing Enabled by Listeriolysin O-liposome-mediated Delivery of the Protein Toxin Gelonin *". Journal of Biological Chemistry. 278 (37): 35102–35108. doi:https://doi.org/10.1074/jbc.M305411200.
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- ^ Shin, Meong Cheol; Zhang, Jian; David, Allan E.; Trommer, Wolfgang E.; Kwon, Young Min; Min, Kyoung Ah; Kim, Jin H.; Yang, Victor C. (28 November 2013). "Chemically and biologically synthesized CPP-modified gelonin for enhanced anti-tumor activity". Journal of Controlled Release. 172 (1): 169–178. doi:https://doi.org/10.1016/j.jconrel.2013.08.016.
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- ^ French, R. R.; Penney, C. A.; Browning, A. C.; Stirpe, F.; George, A. J. T.; Glennie, M. J. (May 1995). "Delivery of the ribosome-inactivating protein, gelonin, to lymphoma cells via CD22 and CD38 using bispecific antibodies". British Journal of Cancer. 71 (5): 986–994. doi:https://doi.org/10.1038/bjc.1995.190.
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