Search results
There is a page named "Primer Dimer" on Wikipedia
- A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...14 KB (1,573 words) - 10:45, 9 July 2024
mishybridization. Primers should not easily anneal with other primers in the mixture; this phenomenon can lead to the production of 'primer dimer' products contaminating...16 KB (2,112 words) - 01:14, 11 April 2024- bands on PCR gels. Primer dimer formation often competes with formation of the DNA fragment of interest, and may be avoided using primers that are designed...16 KB (2,030 words) - 10:07, 20 June 2024
all dsDNA PCR products, including nonspecific PCR products (such as primer dimer). This can potentially interfere with, or prevent, accurate monitoring...43 KB (5,338 words) - 11:09, 12 July 2024- Hot start PCR (section Modified primers)the mixture, are the cause behind the synthesis of by-products such as primer dimer and mis-priming. Mis-priming greatly impedes and reduces the efficiency...22 KB (2,750 words) - 14:49, 30 April 2023
Polymerase chain reaction (redirect from Single Specific Primer-PCR)that utilizes primers with a 3' extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for...89 KB (11,117 words) - 12:33, 8 July 2024
reduced primer dimer formation, detection of alternative splicing variants, ability to perform multiplex PCR with higher numbers of PCR primers, and the...5 KB (596 words) - 01:29, 3 December 2023
facilitates high specificity of the primers and reduces the production of nonspecific products, such as primer dimer. Also, use of a thermostable polymerase...22 KB (2,604 words) - 06:32, 1 June 2024- added to view LAMP in real-time. However, in the late amplification, primer-dimer amplification may contribute to a false positive signal. The use of inorganic...26 KB (3,025 words) - 15:17, 1 July 2024
John Primer (born March 5, 1945, Camden, Mississippi, United States) is an American Chicago blues and electric blues singer and guitarist who played behind...30 KB (2,311 words) - 21:35, 15 June 2024
using DNA binding dyes such as SYBR Green since the elimination of primer-dimers can be achieved through a simple change in the melting temperature....43 KB (5,536 words) - 07:35, 12 July 2024- Variants of PCR (section Primer modifications)PCR (rhPCR) can reduce primer-dimer formation, and increase the number of assays in multiplex PCR. The method utilizes primers with a cleavable block...32 KB (4,102 words) - 05:25, 7 February 2024
- Protein engineering (section Trimer-dimer mutagenesis)which uses only forward primers, while the second reaction uses only reverse primers. This avoids the formation of primer dimer formation.[page needed]...58 KB (7,493 words) - 11:39, 18 July 2024
PCR artifacts (e.g. primer-dimers or truncated products) can increase the background noise and obscure the signal. Primer-dimers can also inhibit the...26 KB (3,176 words) - 03:46, 22 January 2024- not used at all, and is instead replaced with uracil. thymine dimer See pyrimidine dimer. tissue tissue culture tissue-specific gene expression Gene function...254 KB (21,924 words) - 16:54, 4 August 2024
balance, resulting in inefficient ligation and an excess amount of primer dimers, respectively. Therefore, it is important to keep the molar concentration...18 KB (2,276 words) - 12:19, 14 May 2024
reaction. The PCR products are purified again using AMPure beads to remove primer dimers and then quantified before being sequenced. Regions of chromatin that...98 KB (10,972 words) - 12:56, 11 June 2024- news. 2020-09-09. "HostDime obtiene el primer certificado ISO 27701 emitido en Brasil". datacenterdynamics.com. 2020-12-15. "HostDime begins work on Colombian...13 KB (1,094 words) - 17:53, 5 July 2024
- PCR reaction. NetPrimer also analyzes the thermodynamically important secondary structures such as hairpins, self and cross dimers, runs and repeats...4 KB (402 words) - 22:54, 16 May 2024
- Replisome (section Primer removal and nick ligation)asymmetric dimer with DNA polymerase epsilon.) This polymerase synthesizes leading strand DNA in eukaryotes. (Thought to form an asymmetric dimer with DNA...32 KB (3,700 words) - 00:38, 1 January 2024
- followed, with a small quantity of black prismatic powder behind it, as a primer. Then the breech-block was swung into position and locked again, and the
- movie delights me with its cocky confidence that the audience can keep up. Primer is a film for nerds, geeks, brainiacs, Academic Decathlon winners, programmers
- of the primer. Primers should not be self-complementary or complementary to the other primer in the reaction, otherwise they can form dimers. Especially